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  1. \documentclass[a4paper]{tufte-handout}
  2. \title{Protocol number X - Title Y}
  3. %\author[]{Daniel Klapak ,Sarah Broadfoot ,Gregory Penner,Anup Singh ,Eshwar Inapuri [https://doi.org/10.1371/journal.pone.0205460] }
  4. \date{04 April 2022} % without \date command, current date is supplied
  5. \usepackage[utf8]{inputenc}
  6. \usepackage[english]{babel}
  7. \usepackage[sfdefault, light]{FiraSans}
  8. \usepackage[T1]{fontenc}
  9. \usepackage{parskip}
  10. \usepackage{textcomp}
  11. \usepackage{array}
  12. \usepackage{enumitem,amssymb}
  13. \newlist{todolist}{itemize}{2}
  14. \setlist[todolist]{label=$\square$}
  15. \usepackage{graphicx} % allow embedded images
  16. \setkeys{Gin}{width=\linewidth,totalheight=\textheight,keepaspectratio}
  17. \graphicspath{ {./images/} } % set of paths to search for images
  18. \usepackage{amsmath} % extended mathematics
  19. \usepackage{booktabs} % book-quality tables
  20. \usepackage{units} % non-stacked fractions and better unit spacing
  21. \usepackage{multicol} % multiple column layout facilities
  22. \usepackage{lipsum} % filler text
  23. \usepackage{fancyvrb} % extended verbatim environments
  24. \usepackage{multicol}
  25. \setlength{\columnsep}{0.75cm}
  26. \fvset{fontsize=\normalsize}% default font size for fancy-verbatim environments
  27. % Standardize command font styles and environments
  28. \newcommand{\doccmd}[1]{\texttt{\textbackslash#1}}% command name -- adds backslash automatically
  29. \newcommand{\docopt}[1]{\ensuremath{\langle}\textrm{\textit{#1}}\ensuremath{\rangle}}% optional command argument
  30. \newcommand{\docarg}[1]{\textrm{\textit{#1}}}% (required) command argument
  31. \newcommand{\docenv}[1]{\textsf{#1}}% environment name
  32. \newcommand{\docpkg}[1]{\texttt{#1}}% package name
  33. \newcommand{\doccls}[1]{\texttt{#1}}% document class name
  34. \newcommand{\docclsopt}[1]{\texttt{#1}}% document class option name
  35. \newenvironment{docspec}{\begin{quote}\noindent}{\end{quote}}% command specification environment
  36. \begin{document}
  37. \footnotesize
  38. \maketitle% this prints the handout title, author, and date
  39. \vspace{-2em}
  40. \begin{abstract}
  41. \noindent
  42. \footnotesize
  43. Summary of the Current experiment can be provided here in this paragraph
  44. \end{abstract}
  45. \vspace{-2em}
  46. \section{Chemicals used :}
  47. \vspace{-1em}
  48. \begin{table}[h!]
  49. \footnotesize
  50. \centering
  51. \begin{tabular}{||c | c | c | c||}
  52. \hline
  53. Chemical name & Provider & Mol wt.(g/mol) & Additional safety requirements \\ [0.5ex]
  54. \hline\hline
  55. 1 & 6 & 87837 & 787 \\
  56. 2 & 7 & 78 & 5415 \\
  57. 3 & 545 & 778 & 7507 \\
  58. 4 & 545 & 18744 & 7560 \\
  59. 5 & 88 & 788 & 6344 \\ [1ex]
  60. \hline
  61. \end{tabular}
  62. \end{table}
  63. \vspace{-1em}
  64. \section{Experiment}\label{sec:page-layout}
  65. \vspace{-1em}
  66. \begin{multicols}{2}
  67. \subsection{Buffers and solutions}\label{sec:headings}
  68. \begin{todolist}
  69. \item Carboxyl-coated or silica-coated magnetic beads in TE buffer (X µl per sample)\\
  70. \item TE buffer (X µl per sample)\\
  71. \item Isopropanol (X µl per sample) \\
  72. \item 80\% ethanol (X µl per sample) \\
  73. \item Lysis buffer (. X µl per sample) find composition\\
  74. \end{todolist}
  75. \vspace{-1em}
  76. \subsection{Equipment and Setup}\label{sec:sidenotes}
  77. \begin{todolist}
  78. \item Fume hood\\
  79. \item Magnetic stand \\
  80. \item Minifuge \\
  81. \item Heat block \\
  82. \item Pipettes\\
  83. \end{todolist}
  84. \vspace{-1em}
  85. \end{multicols}
  86. \vspace{-1em}
  87. \begin{table}
  88. \centering
  89. \footnotesize
  90. \begin{tabular}{||c | c | c | c||}
  91. \hline
  92. \centering
  93. Reagents &Concentrations & Stock solution & For X ml \\ [1ex]
  94. \hline\hline
  95. GITC & 2 & 3 & 4\\
  96. Tris HCl & 7 & 78 & 5415 \\
  97. Sarkosyl & 545 & 778 & 7507 \\
  98. EDTA & 545 & 18744 & 7560 \\
  99. Antifoam (optional) & 88 & 788 & 6344 \\ [0.5ex]
  100. \hline
  101. \end{tabular}
  102. \end{table}
  103. \subsection{Sample collection and TNA purification}
  104. \vspace{-1em}
  105. \begin{table}[h!]
  106. \footnotesize
  107. \begin{tabular}{| m{12cm} | m{1cm} | m{3 cm}|}
  108. \hline
  109. Step & Time & Observations/Notes \\ [0.1ex]
  110. \hline\hline
  111. \begin{todolist}
  112. \item Collect up to 106
  113. cultured mammalian cells in a single well of a deep-well plate
  114. \end{todolist} & & \\
  115. \begin{todolist}
  116. \item Pellet the cells via centrifugation at 500g and discard supernatant
  117. \end{todolist} & 10 min & \\
  118. \hline
  119. \end{tabular}
  120. \end{table}
  121. \section{Observations}
  122. \vspace{-1em}
  123. Add observations here
  124. \vspace{-1em}
  125. \section{Results}\label{sec:results}
  126. \vspace{-1em}
  127. Add results here
  128. \vspace{-1em}
  129. \section{Iterations}
  130. \vspace{-1em}
  131. Add iterations here
  132. \end{document}