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- \documentclass[a4paper]{tufte-handout}
-
- \title{Protocol number X - Title Y}
-
- %\author[]{Daniel Klapak ,Sarah Broadfoot ,Gregory Penner,Anup Singh ,Eshwar Inapuri [https://doi.org/10.1371/journal.pone.0205460] }
-
- \date{04 April 2022} % without \date command, current date is supplied
-
- \usepackage[utf8]{inputenc}
- \usepackage[english]{babel}
- \usepackage[sfdefault, light]{FiraSans}
- \usepackage[T1]{fontenc}
- \usepackage{parskip}
- \usepackage{textcomp}
- \usepackage{array}
- \usepackage{enumitem,amssymb}
- \newlist{todolist}{itemize}{2}
- \setlist[todolist]{label=$\square$}
-
- \usepackage{graphicx} % allow embedded images
- \setkeys{Gin}{width=\linewidth,totalheight=\textheight,keepaspectratio}
- \graphicspath{ {./images/} } % set of paths to search for images
- \usepackage{amsmath} % extended mathematics
- \usepackage{booktabs} % book-quality tables
- \usepackage{units} % non-stacked fractions and better unit spacing
- \usepackage{multicol} % multiple column layout facilities
- \usepackage{lipsum} % filler text
- \usepackage{fancyvrb} % extended verbatim environments
- \usepackage{multicol}
- \setlength{\columnsep}{0.75cm}
- \fvset{fontsize=\normalsize}% default font size for fancy-verbatim environments
-
-
-
- % Standardize command font styles and environments
- \newcommand{\doccmd}[1]{\texttt{\textbackslash#1}}% command name -- adds backslash automatically
- \newcommand{\docopt}[1]{\ensuremath{\langle}\textrm{\textit{#1}}\ensuremath{\rangle}}% optional command argument
- \newcommand{\docarg}[1]{\textrm{\textit{#1}}}% (required) command argument
- \newcommand{\docenv}[1]{\textsf{#1}}% environment name
- \newcommand{\docpkg}[1]{\texttt{#1}}% package name
- \newcommand{\doccls}[1]{\texttt{#1}}% document class name
- \newcommand{\docclsopt}[1]{\texttt{#1}}% document class option name
- \newenvironment{docspec}{\begin{quote}\noindent}{\end{quote}}% command specification environment
-
- \begin{document}
- \footnotesize
-
- \maketitle% this prints the handout title, author, and date
-
-
- \vspace{-2em}
-
- \begin{abstract}
- \noindent
- \footnotesize
- Summary of the Current experiment can be provided here in this paragraph
- \end{abstract}
-
- \vspace{-2em}
-
- \section{Chemicals used :}
- \vspace{-1em}
- \begin{table}[h!]
- \footnotesize
- \centering
- \begin{tabular}{||c | c | c | c||}
- \hline
- Chemical name & Provider & Mol wt.(g/mol) & Additional safety requirements \\ [0.5ex]
- \hline\hline
- 1 & 6 & 87837 & 787 \\
- 2 & 7 & 78 & 5415 \\
- 3 & 545 & 778 & 7507 \\
- 4 & 545 & 18744 & 7560 \\
- 5 & 88 & 788 & 6344 \\ [1ex]
- \hline
- \end{tabular}
- \end{table}
-
- \vspace{-1em}
-
- \section{Experiment}\label{sec:page-layout}
- \vspace{-1em}
- \begin{multicols}{2}
- \subsection{Buffers and solutions}\label{sec:headings}
- \begin{todolist}
- \item Carboxyl-coated or silica-coated magnetic beads in TE buffer (X µl per sample)\\
- \item TE buffer (X µl per sample)\\
- \item Isopropanol (X µl per sample) \\
- \item 80\% ethanol (X µl per sample) \\
- \item Lysis buffer (. X µl per sample) find composition\\
-
- \end{todolist}
-
-
-
- \vspace{-1em}
-
-
- \subsection{Equipment and Setup}\label{sec:sidenotes}
-
- \begin{todolist}
- \item Fume hood\\
- \item Magnetic stand \\
- \item Minifuge \\
- \item Heat block \\
- \item Pipettes\\
- \end{todolist}
- \vspace{-1em}
-
-
- \end{multicols}
-
- \vspace{-1em}
-
- \begin{table}
- \centering
- \footnotesize
- \begin{tabular}{||c | c | c | c||}
- \hline
- \centering
- Reagents &Concentrations & Stock solution & For X ml \\ [1ex]
- \hline\hline
- GITC & 2 & 3 & 4\\
- Tris HCl & 7 & 78 & 5415 \\
- Sarkosyl & 545 & 778 & 7507 \\
- EDTA & 545 & 18744 & 7560 \\
- Antifoam (optional) & 88 & 788 & 6344 \\ [0.5ex]
- \hline
- \end{tabular}
- \end{table}
-
- \subsection{Sample collection and TNA purification}
-
- \vspace{-1em}
-
- \begin{table}[h!]
- \footnotesize
- \begin{tabular}{| m{12cm} | m{1cm} | m{3 cm}|}
- \hline
- Step & Time & Observations/Notes \\ [0.1ex]
- \hline\hline
- \begin{todolist}
- \item Collect up to 106
- cultured mammalian cells in a single well of a deep-well plate
- \end{todolist} & & \\
- \begin{todolist}
- \item Pellet the cells via centrifugation at 500g and discard supernatant
- \end{todolist} & 10 min & \\
- \hline
- \end{tabular}
- \end{table}
-
-
-
- \section{Observations}
- \vspace{-1em}
- Add observations here
- \vspace{-1em}
- \section{Results}\label{sec:results}
- \vspace{-1em}
- Add results here
- \vspace{-1em}
- \section{Iterations}
- \vspace{-1em}
- Add iterations here
-
-
- \end{document}
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