\documentclass[a4paper]{tufte-handout}

\title{Protocol number X - Title Y}

%\author[]{Daniel Klapak ,Sarah Broadfoot ,Gregory Penner,Anup Singh ,Eshwar Inapuri [https://doi.org/10.1371/journal.pone.0205460] }

\date{04 April 2022} % without \date command, current date is supplied

\usepackage[utf8]{inputenc}
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\usepackage{array}
\usepackage{enumitem,amssymb}
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\setlist[todolist]{label=$\square$}

\usepackage{graphicx} % allow embedded images
  \setkeys{Gin}{width=\linewidth,totalheight=\textheight,keepaspectratio}
\graphicspath{ {./images/} } % set of paths to search for images
\usepackage{amsmath}  % extended mathematics
\usepackage{booktabs} % book-quality tables
\usepackage{units}    % non-stacked fractions and better unit spacing
\usepackage{multicol} % multiple column layout facilities
\usepackage{lipsum}   % filler text
\usepackage{fancyvrb} % extended verbatim environments
\usepackage{multicol}
\setlength{\columnsep}{0.75cm}
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\newenvironment{docspec}{\begin{quote}\noindent}{\end{quote}}% command specification environment

\begin{document}
	\footnotesize

\maketitle% this prints the handout title, author, and date


\vspace{-2em}

\begin{abstract}
\noindent
	\footnotesize
Summary of the Current experiment can be provided here in this paragraph
\end{abstract}

\vspace{-2em}

\section{Chemicals used :}
\vspace{-1em}
\begin{table}[h!]
	\footnotesize
	\centering
	\begin{tabular}{||c | c | c | c||} 
		\hline
		Chemical name & Provider & Mol wt.(g/mol) & Additional safety requirements \\ [0.5ex] 
		\hline\hline
		1 & 6 & 87837 & 787 \\ 
		2 & 7 & 78 & 5415 \\
		3 & 545 & 778 & 7507 \\
		4 & 545 & 18744 & 7560 \\
		5 & 88 & 788 & 6344 \\ [1ex] 
		\hline
	\end{tabular}
\end{table}

\vspace{-1em}

\section{Experiment}\label{sec:page-layout}
\vspace{-1em}
\begin{multicols}{2}
\subsection{Buffers and solutions}\label{sec:headings}
\begin{todolist}
\item Carboxyl-coated or silica-coated magnetic beads in TE buffer (X µl per sample)\\
\item TE buffer (X µl per sample)\\
\item Isopropanol (X µl per sample) \\
\item 80\% ethanol (X µl per sample) \\
\item Lysis buffer (. X µl per sample) find composition\\

\end{todolist}



\vspace{-1em}


\subsection{Equipment and Setup}\label{sec:sidenotes}

\begin{todolist}
\item Fume hood\\
\item Magnetic stand \\
\item Minifuge \\
\item Heat block \\
\item Pipettes\\
\end{todolist}
\vspace{-1em}


\end{multicols}

\vspace{-1em}

\begin{table}
	\centering
	\footnotesize
	\begin{tabular}{||c | c | c | c||} 
	\hline
	\centering
	Reagents &Concentrations & Stock solution & For X ml \\ [1ex] 
	\hline\hline
	GITC & 2 & 3 & 4\\ 
	Tris HCl & 7 & 78 & 5415 \\
	Sarkosyl & 545 & 778 & 7507 \\
	EDTA & 545 & 18744 & 7560 \\
	Antifoam (optional) & 88 & 788 & 6344 \\ [0.5ex] 
	\hline
	\end{tabular}
\end{table}

\subsection{Sample collection and TNA purification}

\vspace{-1em}

 \begin{table}[h!]
 	\footnotesize
 	\begin{tabular}{|  m{12cm} | m{1cm} | m{3 cm}|} 
 		\hline
 	 Step & Time & Observations/Notes \\ [0.1ex] 
 		\hline\hline
\begin{todolist}
	\item Collect up to 106
 		cultured mammalian cells in a single well of a deep-well plate
 		\end{todolist} & &  \\ 
\begin{todolist} 
	\item Pellet the cells via centrifugation at 500g and discard supernatant
	 	\end{todolist} & 10 min & \\
 		\hline
 	\end{tabular}
 \end{table}



\section{Observations}
\vspace{-1em}
Add observations here  
\vspace{-1em}
\section{Results}\label{sec:results}
\vspace{-1em}
Add results here
\vspace{-1em}
\section{Iterations}
\vspace{-1em}
Add iterations here	


\end{document}
